Formation of the photosynthetic surface of sunflower lines depending on genotype and pre-sowing seed treatment with growth stimulants and micronutrients
DOI:
https://doi.org/10.31359/2413.7642.2026.1.135Keywords:
соняшник, фотосинтетичний потенціал, лінія, стимулятори росту рослин, мікродобрива, передпосівна обробка насінняAbstract
Formulation of the problem. Increasing sunflower productivity largely depends on the efficiency of the photosynthetic apparatus, particularly the leaf area, which determines the intensity of assimilatory processes. At the same time, its formation is governed by both genotypic characteristics and agronomic practices, among which pre-sowing seed treatment with growth stimulants and micronutrients plays an important role. The insufficient study of their effects on different sunflower lines under the conditions of the Eastern Forest-Steppe of Ukraine determines the relevance of this research. Purpose. To determine the peculiarities of the formation of the photosynthetic surface of sunflower lines depending on different variants of pre-sowing seed treatment and to assess their impact on yield. Methods. Field experiments were conducted during 2022–2024 on typical medium-humus slightly leached chernozem soils. The objects of the study were sunflower lines Sх17A, Sх51A, and Sх808A. The experimental design included various variants of pre-sowing seed treatment using seed dressers, growth stimulants, and micronutrients. Leaf area and yield were measured, and analysis of variance, correlation, and regression analyses were applied. Results. It was established that the photosynthetic surface significantly varied depending on genotype and seed treatment variant. The highest values were observed in line Sх808A, while the lowest were recorded in Sх51A. The application of growth stimulants and micronutrients generally increased leaf area, with the most effective treatments involving Nertus Plantapeg, Nertus Start, and Raikat Start. According to the analysis of variance, the contribution of genotype was 77%, year conditions – 8%, and seed treatment – 3%. A strong positive correlation between leaf area and yield was revealed (r = 0.920; R² = 0.846). The greatest yield increase was obtained in treatments with Nertus Plantapeg and AKM. Conclusions. The formation of the photosynthetic surface of sunflower lines is mainly determined by the genotype, which has a dominant effect, while pre-sowing seed treatment and year conditions have a smaller but statistically significant effect. The regression coefficient shows that an increase in leaf surface area by 1 thousand m²/ha is accompanied by an increase in yield by an average of 0.055 t/ha.
References
Список використаних джерел
1. Valentim S. M. S., Luz P. B. D., Moreira A. C. S., Cardoso E. L. O., Moretto G., Tomiozzo R. Thermal sum and phyllochron of cut sunflower genotypes in southwestern Mato Grosso, Brazil. Ciência e Agrotecnologia. 2025. Vol. 49. e017024. https://doi.org/10.1590/1413-7054202549017024
2. dos Santos E. R., Barros H. B., Capone A., de Castro Ferraz E., Fidelis R. R. Effects of sowing periods on sunflower cultivars in the South of the State of Tocantins. Revista Ciência Agronômica. 2012. Vol. 43, No. 1. P. 199. https://doi.org/10.1590/S1806-66902012000100025
3. Грицаєнко З. М., Карпенко В. П., Підан Л. Ф. Стан фотосинтетичної та пігментної систем соняшника за дії гербіцидів Фюзилад форте 150, Дуал голд 960 та регулятора росту рослин Радостим. Збірник наукових праць Уманського національного університету садівництва. 2014. № 86 (1). С. 221–228.
4. Kalenska S., Ryzhenko A., Novytska N., Garbar L., Stolyarchuk T., Kalenskyi V., Shytiy O. Morphological features of plants and yield of sunflower hybrids cultivated in the Northern part of the Forest-Steppe of Ukraine. American Journal of Plant Sciences. 2020. Vol. 11, No. 8. P. 1331–1344. https://doi.org/10.4236/ajps.2020.118095
5. Lecoeur J., Vincourt P., Vear F. Les possibilités de sélection pour le rendement en grains du tournesol par l’étude de caractères morphologiques et architecturaux. Oléagineux, Corps gras, Lipides. 2010. Vol. 17, No. 3. P. 139–142. https://doi.org/10.1684/OCL.2010.0305
6. Чуйко Д. В., Кириченко В. В., Білик В. В. Агробіологічна оцінка гібридів соняшнику в умовах Східної України. Селекція і насінництво. 2025. № 127. С. 56–67. https://doi.org/10.30835/2413-7510.2025.333766
7. Domaratskyi Y. Leaf area formation and photosynthetic activity of sunflower plants depending on fertilizers and growth regulators. Journal of Ecological Engineering. 2021. Vol. 22, No. 6. P. 95–105. http://dx.doi.org/10.12911/22998993/137361
8. Sakhoshko M. M., Kravchenko M. I., Yatsenko V. M., Kolosok I. O. Development of the leaf area and the productivity structure of the sunflower hybrids in the North-Eastern Forest-Steppe of Ukraine. Bulletin of Sumy National Agrarian University. The series: Agronomy and Biology. 2019. No. 1–2 (35–36). P. 33–39. https://doi.org/10.32845/agrobio.2019.1-2.5
9. Hladni N., Škorić D., Kraljević-Balalić M. Genetic variance of sunflower yield components (Helianthus annuus L.). Genetika-Belgrade. 2003. Vol. 35, No. 1. P. 1–9. https://doi.org/10.2298/GENSR0301001H
10. Chuiko D. Plant growth regulator effects on sunflower parents and F1 hybrids. Žemės ūkio mokslai. 2021. Vol. 28, No. 2. https://doi.org/10.6001/zemesukiomokslai.v28i2.4508
11. Fernández Moroni I., Fraysse M., Presotto A. D., Cantamutto M. Á. Evaluation of Argentine wild sunflower biotypes for drought stress during reproductive stage. Helia. 2012. Vol. 35, No. 57. P. 29–36. https://doi.org/10.2298/hel1257029f
12. Чуйко Д. В. Продуктивність і елементи формування структури урожаю генотипів соняшнику при обробці регуляторами росту рослин. Вісник ХНАУ. Серія: Рослинництво, селекція і насінництво, плодоовочівництво. 2020. № 1–2. С. 114–127. https://doi.org/10.35550/visnykagro2020.01-02.114
13. Khan M. A., Sharma V., Shukla R. K. Response of sunflower (Helianthus annuus L.) to organic manure and biofertilizer under different levels of mycorrhiza and sulphur in comparison with inorganic fertilizer. Journal of Crop and Weed. 2016. Vol. 12, No. 1. P. 81–86.
14. Kumari S. Effects of nitrogen levels on anatomy, growth, and chlorophyll content in sunflower (Helianthus annuus L.) leaves. Journal of Agricultural Science. 2017. Vol. 9, No. 8. P. 208–219. https://doi.org/10.5539/jas.v9n8p208
15. Seabra Filho M., Menezes A. S., Neto L. G. P., de Azevedo B. M., de Araújo Viana T. V. Effects of split-applied nitrogen fertigation on sunflower (Helianthus annuus). REVISTA DELOS. 2023. Vol. 16, No. 44. P. 1402–1421. https://doi.org/10.55905/rdelosv16.n44-026
16. Hanhur V. V., Yeremko L. S., Kocherha A. A. Ефективність біостимуляторів за умови передпосівної обробки насіння соняшнику. Scientific Progress & Innovations. 2020. No. 2. P. 36–42. https://doi.org/10.31210/visnyk2020.02.04
17. Naik K. B., Nataraj S. K., Shadakshari Y. G., Kumar D. P., Seetharamu G. K., Jayaprasad K. V. Effect of pre harvest foliar spray of growth regulators on pre and post harvest parameters in ornamental sunflower genotype M-17R. Journal of Horticultural Sciences. 2018. Vol. 13, No. 1. P. 48–53. https://doi.org/10.24154/JHS.2018.V13I01.005
18. Tsyliuryk O. I., Horshchar V. I., Izhboldin O. O., Kotchenko M. V., Rumbakh M. Y., Hotvianska A. S., Chornobai V. H. The influence of biological products on the growth and development of sunflower plants (Helianthus annuus L.) in the northern steppe of Ukraine. Ukrainian Journal of Ecology. 2021. Vol. 11, No. 3. P. 106–116. https://doi.org/10.31867/2523-4544/0209
19. Волкодав В. В. Методика державного сортовипробування сільськогосподарських культур. Київ: Державна комісія України по випробуванню та охороні сортів рослин, 2000. 100 с.
20. ДСТУ 4138-2002. Насіння сільськогосподарських культур. Методи визначення якості. Київ: Держстандарт України, 2003. 173 с.
21. Спеціальна селекція і насінництво польових культур : навч. посіб. / за ред. В. В. Кириченка. Харків: ІР ім. В. Я. Юр’єва НААН, 2010. 462 с.
22. Hammer Ø., Harper D. A. Past: paleontological statistics software package for education and data analysis. Palaeontologia Electronica. 2001. Vol. 4, No. 1. P. 1.
23. Чуйко Д. В. Формування підвищеної продуктивності генотипів соняшнику залежно від регуляторів росту рослин в східній частині Лісостепу України: дис. … д-ра філософії: 201 – Агрономія. Харків: Державний біотехнологічний університет, 2021. 220 с.
REFERENCES
1. Valentim, S. M. S., Luz, P. B. D., Moreira, A. C. S., Cardoso, E. L. O., Moretto, G., & Tomiozzo, R. (2025). Thermal sum and phyllochron of cut sunflower genotypes in southwestern Mato Grosso, Brazil. Ciência e Agrotecnologia, 49, e017024. https://doi.org/10.1590/1413-7054202549017024
2. dos Santos, E. R., Barros, H. B., Capone, A., de Castro Ferraz, E., & Fidelis, R. R. (2012). Effects of sowing periods on sunflower cultivars in the South of the State of Tocantins. Revista Ciência Agronômica, 43(1), 199. https://doi.org/10.1590/S1806-66902012000100025
3. Hrytsaienko, Z. M., Karpenko, V. P., & Pidan, L. F. (2014). Photosynthetic and pigment system state of sunflower under the action of herbicides Fusilade Forte 150, Dual Gold 960 and plant growth regulator Radostim. Collection of Scientific Works of Uman National University of Horticulture, 86(1), 221–228.
4. Kalenska, S., Ryzhenko, A., Novytska, N., Garbar, L., Stolyarchuk, T., Kalenskyi, V., & Shytiy, O. (2020). Morphological features of plants and yield of sunflower hybrids cultivated in the Northern part of the Forest-Steppe of Ukraine. American Journal of Plant Sciences, 11(8), 1331–1344. https://doi.org/10.4236/ajps.2020.118095
5. Lecoeur, J., Vincourt, P., & Vear, F. (2010). Les possibilités de sélection pour le rendement en grains du tournesol par l’étude de caractères morphologiques et architecturaux. Oléagineux, Corps gras, Lipides, 17(3), 139–142. https://doi.org/10.1684/OCL.2010.0305
6. Chuiko, D. V., Kyrychenko, V. V., & Bilyk, V. V. (2025). Agrobiological evaluation of sunflower hybrids in Eastern Ukraine. Plant Breeding and Seed Production, 127, 56–67. https://doi.org/10.30835/2413-7510.2025.333766
7. Domaratskyi, Y. (2021). Leaf area formation and photosynthetic activity of sunflower plants depending on fertilizers and growth regulators. Journal of Ecological Engineering, 22(6), 95–105. http://dx.doi.org/10.12911/22998993/137361
8. Sakhoshko, M. M., Kravchenko, M. I., Yatsenko, V. M., & Kolosok, I. O. (2019). Development of the leaf area and the productivity structure of the sunflower hybrids in the North-Eastern Forest-Steppe of Ukraine. Bulletin of Sumy National Agrarian University. The Series: Agronomy and Biology, 1-2(35–36), 33–39. https://doi.org/10.32845/agrobio.2019.1-2.5
9. Hladni, N., Škorić, D., & Kraljević-Balalić, M. (2003). Genetic variance of sunflower yield components—Helianthus annuus L. Genetika-Belgrade, 35(1), 1–9. https://doi.org/10.2298/GENSR0301001H
10. Chuiko, D. (2021). Plant growth regulator effects on sunflower parents and F1 hybrids. Žemės ūkio mokslai, 28(2). https://doi.org/10.6001/zemesukiomokslai.v28i2.4508
11. Fernández Moroni, I., Fraysse, M., Presotto, A. D., & Cantamutto, M. Á. (2012). Evaluation of Argentine wild sunflower biotypes for drought stress during reproductive stage. Helia, 35(57), 29–36. https://doi.org/10.2298/hel1257029f
12. Chuiko, D. V. (2020). Productivity and elements of sunflower yield structure formation under plant growth regulators treatment. Visnyk KhNAU. Series: Crop Production, Breeding and Seed Production, Horticulture, 1-2, 114–127. https://doi.org/10.35550/visnykagro2020.01-02.114
13. Khan, M. A., Sharma, V., & Shukla, R. K. (2016). Response of sunflower (Helianthus annuus L.) to organic manure and biofertilizer under different levels of mycorrhiza and sulphur in comparison with inorganic fertilizer. Journal of Crop and Weed, 12(1), 81–86.
14. Kumari, S. (2017). Effects of nitrogen levels on anatomy, growth, and chlorophyll content in sunflower (Helianthus annuus L.) leaves. Journal of Agricultural Science, 9(8), 208–219. https://doi.org/10.5539/jas.v9n8p208
15. Seabra Filho, M., Menezes, A. S., Neto, L. G. P., de Azevedo, B. M., & de Araújo Viana, T. V. (2023). Effects of split-applied nitrogen fertigation on sunflower (Helianthus annuus). REVISTA DELOS, 16(44), 1402–1421. https://doi.org/10.55905/rdelosv16.n44-026
16. Hanhur, V. V., Yeremko, L. S., & Kocherha, A. A. (2020). Efficiency of biostimulants under pre-sowing treatment of sunflower seeds. Scientific Progress & Innovations, 2, 36–42. https://doi.org/10.31210/visnyk2020.02.04
17. Naik, K. B., Nataraj, S. K., Shadakshari, Y. G., Kumar, D. P., Seetharamu, G. K., & Jayaprasad, K. V. (2018). Effect of pre-harvest foliar spray of growth regulators on pre- and post-harvest parameters in ornamental sunflower genotype M-17R. Journal of Horticultural Sciences, 13(1), 48–53. https://doi.org/10.24154/JHS.2018.V13I01.005
18. Tsyliuryk, O. I., Horshchar, V. I., Izhboldin, O. O., Kotchenko, M. V., Rumbakh, M. Y., Hotvianska, A. S., … Chornobai, V. H. (2021). The influence of biological products on the growth and development of sunflower plants (Helianthus annuus L.) in the northern steppe of Ukraine. Ukrainian Journal of Ecology, 11(3), 106–116. https://doi.org/10.31867/2523-4544%2F0209
19. Volkodav, V. V. (2000). Methodology of state variety testing of agricultural crops. Kyiv: State Commission of Ukraine for Testing and Protection of Plant Varieties.
20. DSTU 4138-2002. (2003). Seeds of agricultural crops. Methods for determining quality [Official ed.]. Kyiv: Derzhstandart of Ukraine.
21. Kyrychenko, V. V. (Ed.). (2010). Special breeding and seed production of field crops: textbook. Kharkiv: V. Ya. Yuriev Institute, NAAS of Ukraine.
22. Hammer, Ø., & Harper, D. A. (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4(1), 1.
23. Chuiko, D. V. (2021). Formation of increased productivity of sunflower genotypes depending on plant growth regulators in the eastern part of the Forest-Steppe of Ukraine (Doctoral dissertation, State Biotechnology University, Ukraine).